DNA Extraction and Purification
Macherey-Nagel NucleoBond Xtra Maxi, 100 preps
NucleoBond Xtra Maxi (100) 100 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Spleen, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Enzo Life Sciences pJY2 plasmid (5µg)
The AGA arginine codon is rarely used in E. coli but is common in eukaryotic genes. The low level of tRNAUCUArg can lead to low expression and misincorporation of lysine for arginine, during expression of genes containing AGA codons in E. coli. E. coli harboring pJY2 can be employed to suppress lysine misincorporation and achieve high-level expression of genes cloned into pETvectors without modification of AGA codons in the target gene sequence. pJY2 encodes tRNAUCUArg (to suppress lysine misincorporation at AGA codons) and T7 lysozyme (to depress constitutive expression of the cloned gene). For example, pJY2 allows for faithful expression of pET3a-encoded mutant ubiquitins with a high density of AGA codons (up to 14% of total codons). pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase. Formulation: Liquid. In 10mM TRIS, pH 8.0, containing 1mM EDTA. Long Term Storage: -20°C.
LGC GENOMICS LLC (Lucigen) sbeadex™ PCR Clean-up Kit, 960 purificationsHighly efficient, magnetic bead based purification of PCR products.
Highly efficient, magnetic bead based purification of PCR products.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Stomach, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
LGC GENOMICS LLC (Lucigen) sbeadex™ PCR Clean-up Kit, 96 purificationsHighly efficient, magnetic bead based purification of PCR products.
Highly efficient, magnetic bead based purification of PCR products.
Biochain Institute Inc Genomic DNA - Human Tumor Tissue: Stomach Tumor, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Magbio Genomics Inc HighPrep DTR (250mL)
HighPrep DTR is a high performance paramagnetic bead-based system for Big Dye sequencing clean up. Designed to remove unincorporated dye terminators from Sanger sequencing reaction, the HighPrep DTR system consists of a selective binding of DNA to the HighPrep DTR paramagnetic beads, followed with washing off nucleotides, primers and non-targeted amplicons, and finally elution of pure DNA. HighPrep DTR is designed for both manual and fully automated purification of sequencing products. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol.
